This article argues that Luke’s Gospel develops an Isaac typology by means of Genesis allusions, especially to the Akedah passage (Gen 22:1–19). First, the methodology with regard to the criteria for detecting allusions is reviewed and the well-recognized example of Elijah typology is considered in order to understand some characteristics of Luke’s use of typology. The question of Isaac typology is then studied, specifically as it is prepared in the infancy narrative, developed with the references to the “beloved son” (cf. Gen 22:2, 12, 16 LXX) at the baptism (Luke 3:22) and in the parable of the wicked tenants (20:13), and completed in the crucifixion passage (23:33; cf. Gen 22:9 LXX). In conclusion, some implications are briefly considered, such as concerning the significance of Jesus’s death in Luke’s Gospel.